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Aspergillus penicillioides

Aspergillus penicillioides

분류체계 : Fungi균계 > Ascomycota > Eurotiomycetes > Eurotiales > Aspergillaceae > Aspergillus >

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    종설명(Description)

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    공간정보

    염기서열정보(Sequence)

    NCBI (Aspergillus penicillioides)

    CaM

    ITS

    염기서열

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        제목내용저자저널명/발행자상세보기
        Characterization of the Proteolytic Activity of a Halophilic Aspergillus reticulatus Strain SK1-1 Isolated from a Solar Saltern

        Salterns are hypersaline environments that are inhabited by diverse halophilic microorganisms, including fungi. In this study, we isolated a fungal strain SK1-1 from a saltern in the Republic of Korea, which was identified as Asperillus reticulatus. This is the first reported saline-environment-derived A. reticulatus that belongs to the Aspergillus penicillioides clade and encompasses xerophilic fungi. SK1-1 was halophilic, obligately requiring NaCl for growth, with a maximum radial growth of 6%？9% (w/v) NaCl. To facilitate the biotechnological application of halophilic fungi, we screened the SK1-1 strain for proteolytic activity. Proteases have widespread applications in food processing, detergents, textiles, and waste treatment, and halophilic proteases can enable protein degradation in high salt environments. We assessed the proteolytic activity of the extracellular crude enzyme of SK1-1 using azocasein as a substrate. The crude protease exhibited maximum activity at 40？50 °C, pH 9.5？10.5, and in the absence of NaCl. It was also able to retain up to 69% of its maximum activity until 7% NaCl. Protease inhibitor assays showed complete inhibition of the proteolytic activity of crude enzymes by Pefabloc® SC. Our data suggest that the halophilic A. reticulatus strain SK1-1 produces an extracellular alkaline serine protease.

        		 Chung, Dawoon,Yu, Woon-Jong,Lim, Ji-Yeon,Kang, Nam-Seon,Kwon, Yong-Min,Choi, Grace,Bae, Seung-Sub,Cho, Kichul,Lee, Dae-Sung MDPI 상세보기

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      MABIK FU00001322 국립해양생물자원관 미생물배양체 Aspergillus penicillioides 태평양 2022-12-13 10 Vial
      MABIK PN46230011 국립해양생물자원관 gDNA Aspergillus penicillioides 태평양 3 Vial
      MABIK NP50240045 국립해양생물자원관 추출물 Aspergillus penicillioides 태평양 2022-12-13 10 Tube
      SFC20231003-M06 해양균류자원 미생물배양체 Aspergillus penicillioides 대한민국 경상북도 울릉군 북면 현포2길 121 울릉도독도해양자원연구센터(KIOST) 앞 2023-07-12 3 Vial
      SFC20250529-M022 해양균류자원 미생물배양체 Aspergillus penicillioides 태평양 2023-12-10 11 Vial
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