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지식채널
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논문정보
검색결과 2건
논문정보 목록 - 제목, 내용, 저자, 저널명/발행자, 상세보기 제목 내용 저자 저널명/발행자 상세보기 The complete mitogenome of Callista chinensis (Bivalvia: Veneridae) Abstract
The complete mitochondrial genome of Callista chinensis was sequenced via next-generation sequencing. The circular genome was 19,704 bp in length, containing 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a putative control region. The gene order of nad2 and nad4l was reversed when compared with that of other Veneridae species. The phylogenetic analysis indicated that the C. chinensis was clustered with Saxidomus purpurata. Comparing nucleotide sequences of the partial cox1 gene from 40 C. chinensis individuals displayed high levels of genetic diversity in the analyzed populations. Additionally, demographic history analysis based on neutrality tests and mismatch distributions suggested a recent population expansion in the C. chinensis.
Li, Biquan,Luo, Site TaylorFrancis 상세보기 Isolation, identification and pathogenesis study of Vibrio diabolicus Abstract
A strain of Vibrio diabolicus named SX-2018 was isolated from Callista chinensis in the Zhejiang coastal region of China. The isolate was identified as V. diabolicus by sequencing of the 16S rRNA, housekeeping genes and subclade-associated genes. Antibiotic resistance as well as biochemical and growth characteristics were determined. Animal studies were performed to evaluate the in vivo virulence of SX-2018. The results indicated that SX-2018 was highly related to the known deep-sea hydrothermal vent V. diabolicus and V. diabolicus from Homo sapiens. Artificial infection showed that SX-2018 exhibited strong pathogenicity toward Gomphina aequilatera, but it did not express pathogenicity toward Mactra veneriformis Reeve, Callista chinensis, Mactra chinensis Philippi, Ruditapes philippinarum, Cyclina sinensis, Dosinia corrugate Meretrix meretrix, Tegillarca granosa, Sinonovacula constricta or Danio rerio. Mortality in Gomphina aequilatera at 25 °C and 15 °C was 100% and 27%, respectively. Virulence-related gene sequence analysis showed that SX-2018 encoded the tlh gene but lacked tdh and trh genes. Tlh and hemolysin coregulated protein genes of SX-2018 shared 99.36?99.79% homology to the deep-sea V. diabolicus strain, 98.65.36?99.79% homology to the V. diabolicus strain from Homo sapiens, and 97?97.79% and 84?89.79% similarity to Vibrio alginolyticus and Vibrio parahaemolyticus, respectively. This study will aid in understanding the evolutionary relationship between V. diabolicus and related pathogenic Vibrio species.
Highlights
- A novel strain of Vibrio diabolicus from Callista chinensis in China was identified.
- The Vibrio diabolicus SX-2018 was highly related to V. diabolicus strains from deep-sea hydrothermal vent and Homo sapiens.
- The Vibrio diabolicus SX-2018 had strong pathogenicity to Gomphina aequilatera.
- Mortality in Gomphina aequilatera at 25°C and 15°C was 100% and 27%, respectively.
Song, Jiayao,Liu, Xiaoyu,Wu, Cuiling,Zhang, Yu,Fan, Ke,Zhang, Xiaodong,Wei, Yongwei Elsevier 상세보기
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